$ 40.00. pUC18 is a commonly used plasmid cloning vector in E. coli. The molecule is a double-stranded circular DNA (2686 base pairs in length). Due to a small size pUC18 enables successful cloning of large DNA fragments.
What does a plasmid map tell you?
Plasmid maps are graphical representation of plasmids, that show the locations of major identifiable landmarks on DNA like restriction enzyme sites, gene of interest, plasmid name and length etc.
What gene is in the pUC18 vector?
pUC18 is a small, high copy cloning vector for replication in E. coli. It has been constructed using the ampicillin resistance gene and the pMB1 origin of replication from pBR322. The pMB1 of pUC18 differs from the pBR322 origin by a single point mutation and the lack of the rop gene, leading to a high copy number.
What is the difference between pUC18 and pUC19?
pUC19 is identical to pUC18 except that they contain multiple cloning sites (MCS) arranged in opposite orientations. pUC18/19 plasmids contain: The pMB1 replicon rep responsible for the replication of plasmid (source – plasmid pBR322).
What is the size of pUC18?
2686 bp
Thermo Scientific pUC18 vector is a small, high copy number, E. coli plasmid, 2686 bp in length. It contains identical multiple cloning site (MCS) as pUC19 vector except that it is arranged in opposite orientation.
What is the function of lacZ in the plasmid pUC18?
Naturally-occurring plasmids are viruses of bacteria. The artificial plasmid pUC18 has been genetically engineered to include (1) a gene for antibiotic resistance to Ampicillin (amp R), and (2) a gene (and its promoter) for the enzyme beta-galactosidase (lacZ).
Why are plasmid maps important?
Mapping of DNA restriction sites is an important part of working in a molecular biotechnology lab because such maps are used to plan cloning strategy and to verify when a DNA clone has been successfully constructed. These data show that each enzyme has only one restriction site within the plasmid.
How do plasmid maps work?
Working a DNA into mapping is done using restriction endonuclease enzymes that are found in bacteria, to cut the DNA into fragments. These enzymes cut specific recognition sites of a DNA molecule which may result in a sticky end i.e. over hangs using EcoR1 in the 5’G-AATTC 3′.
Is pUC18 a expression vector?
These vectors provide novel cloning possibilities in addition to simple, non-expensive, high level expression of recombinant proteins in E. coli. Vectors pUC18 and pUC19 are small high-copy number plasmids that are widely used for cloning and manipulation of DNA fragments (9).
Why is pUC18 used?
Vectors pUC18 and pUC19 are small high-copy number plasmids that are widely used for cloning and manipulation of DNA fragments (9).
How many base pairs are in pUC18?
2686 base pairs
pUC18 is a commonly used plasmid cloning vector in E. coli. The molecule is double-stranded circle, 2686 base pairs in length, and has a high copy number.
What is the size of pUC18 plasmid?
Thermo Scientific pUC18 vector is a small, high copy number, E. coli plasmid, 2686 bp in length. It contains identical multiple cloning site (MCS) as pUC19 vector except that it is arranged in opposite orientation.
