The chicken chorioallantoic membrane (CAM) is a simple, highly vascularized extraembryonic membrane, which performs multiple functions during embryonic development, including but not restricted to gas exchange.
What is the role of Chorioallantoic membrane?
The chick embryo chorioallantoic membrane (CAM) is an extraembryonic membrane. Its main function is to exchange gases and nutrients, which is supported by a dense capillary network 1-3. Due to its extensive vascularization and its ease of use, the CAM is a widely utilized research tool.
Where is the Chorioallantoic membrane?
The Chorioallantoic Membrane (CAM), also known as the chorioallantois, is a highly vascularized membrane found in the eggs of certain amniotes like birds and reptiles. It is formed by the fusion of the mesodermal layers of two extra-embryonic membranes – the chorion and the allantois.
What is Chorioallantoic membrane CAM assay?
One potential approach to refinement animal experimentation is the use of the chorioallantoic membrane (CAM) assay, which involves the implantation of a material or compound on the extraembryonic membrane of the developing chick egg. Critically the CAM is not innervated and thus no pain is experienced by the chick.
Why is the Chorioallantoic membrane used in embryology experiments?
The chick embryo chorioallantoic membrane (CAM) is an extraembryonic membrane. Its main function is to exchange gases and nutrients, which is supported by a dense capillary network [1-3]. Due to its extensive vascularization and its ease of use, the CAM is a widely utilized research tool.
What does the chorion do?
The chorion has two main functions: protect the embryo and nurture the embryo. To protect the embryo, the chorion produces a fluid known as chorionic fluid. The chorionic fluid lies in the chorionic cavity, which is the space between the chorion and the amnion.
What does the chorion develop from?
The chorion is derived from trophoblastic ectoderm and extraembryonic mesoderm (somatopleure). There is an intimate association between the forming chorion and amnion. These form by folding in domestic animals and by so-called cavitation in humans, mice, and rats.
How do you do a CAM assay?
While there have been many variations developed over the years, the basic assay is performed by implanting a membrane or coverslip containing the compound of interest on the chick embryo chorioallantoic membrane through a hole cut in the egg shell.
What does the Chorion do?
What is chorion membrane?
Anatomical terminology. The chorion is the outermost fetal membrane around the embryo in mammals, birds and reptiles (amniotes). It develops from an outer fold on the surface of the yolk sac, which lies outside the zona pellucida (in mammals), known as the vitelline membrane in other animals.
What day does the chorion form?
With growth of the fetus and amniotic cavity, these layers fill the entire uterine cavity by approximately 15 weeks of human pregnancy, forming the attachment of the chorion with the decidua.
