Tyramide signal amplification (TSA), sometimes called Catalyzed Reporter Deposition (CARD), is a highly sensitive method enabling the detection of low-abundance targets in fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC), and in situ hybridization (FISH) applications.
How does TSA amplification work?
Signal amplification in TSA is enabled via binding of biotinylated tyramine to proteins near the site of peroxidase-labeled antibodies. The incubation of the labeled tissue with biotinylated tyramine and hydrogen peroxide (H2O2) results in a peroxide enzyme catalyzed reaction that adds radicals to tyramine.
What is Fluor™ 488 tyramide reagent?
Alexa Fluor™ 488 Tyramide Reagent is a component of SuperBoost™ tyramide signal amplification kits made available separately here. This reagent can be used with any antibody conjugated to HRP for tyramide signal amplification for detection of low abundance targets in multiplexable fluorescent immunocytochemistry (ICC),…
What are the applications of Tyramide signal amplification?
It can be readily integrated into any application that utilizes horseradish peroxidase (HRP) in its protocol. These applications include ELISA, immunocytochemistry (ICC), immunohistochemistry (IHC) and in situ hybridization (FISH). Advantages of tyramide signal amplification, include:
How do you label tyramide?
Tyramide can be labeled with a fluorophore or a hapten (such as biotin or DNP). Multiple rounds of tyramide signal amplification can be performed for multicolor detection (see Figure 2 and our Tech Tip: Multicolor Fluorescence Imaging using Tyramide Amplification Kits ).
What is tyramide amplification buffer plus?
Tyramide Amplification Buffer Plus has enhanced sensitivity for tyramide signal amplification. It has improved brightness, specificity, and sensitivity over our original buffer below. Ready-to-Use Tyramide Amplification Buffer was our original buffer formulation, and has been replaced with the above buffer.
