The 16 gauge needles are the most popular and are recommended if you are making mycelium culture syringes, while the smaller 18 gauge needles are recommended if you are making mushroom spore syringes. We also offer super wide-bore 14 gauge needles used to draw up extra-thick mycelial liquid culture.
Is liquid culture same as spore syringe?
Spore syringes are often mistaken for liquid culture. Liquid culture is the result of an isolated mycelium culture grown and expanded in a sugar broth. Liquid culture is preferred over a spore syringe to inoculate grain spawn due to the reduced chance of contamination.
How do you make a liquid culture from a spore syringe?
Turn one spore syringe into 20 or more! Simply inject 3-5 cc’s of your favorite spores in the culture jar and watch it transform into liquid mycelium. In a few days it will be ready to inject into any substrate jar or spawn bag. It will cut down your colonization times by over a week!
How much of my spore syringe do I use?
You will only require drops of 3 milliliters of the spore solution to colonize 1.05 quart or 1 liter of the substrate. One syringe has 20 ml of the mushroom spore solution which is enough to inoculate between 6 and 7 liters of substrate.
How do you use a liquid culture syringe?
Take new needle out of the bag (leave needle plastic cover on) and connect to syringe. Once the heat source is ready, remove needle plastic cover to expose needle. Squirt three or four drops out of the needle to have liquid in the needle. Heat the tip of needle until glowing red.
How long does liquid culture take to colonize?
Ensure there is enough space between the filter, the plastic, and the grain to allow for air exchange. It doesn’t matter if it is light or dark. Within 3 to 10 days after inoculation you should see signs of growth. After 3 or 4 weeks, your mycelium should have colonized 70 to 75% of the bag.
How much grain should I liquid culture?
Usually about 1 – 2 CC’s of liquid culture is sufficient per quart sized grain jar. You can also inoculate the grain jar without even opening the lid, by simply injecting the liquid culture right though the filter on the top of the jar.
How do you attach a needle to a spore syringe?
Hold the syringe with the cap pointing up. Unscrew the the cap from the spore syringe by turning it counter clockwise. Remove the needle from it’s sterile package (leave the protection cap on the needle) and attach it to the spore syringe.
How many ml is a bag of spores?
We recommend to use about 10 -‐ 20 ml – that is 1 -‐ 2 syringes -‐ for small bags (2,5 L content). For larger bags (4,5 L content) we recommend to use 20 -‐ 30 ml spore solution – that is 2 -‐ 3 spore syringes.
